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dmem f12  (ATCC)


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    Structured Review

    ATCC dmem f12
    Dmem F12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+f12/DMEM%3A+F-12+Medium/pm42277434-325-5-6
    Average 99 stars, based on 5683 article reviews
    dmem f12 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Hyaluronic acid-poly (vinyl alcohol) composite hydrogels with self-assembled peptide nanofibers as Bruch's membrane mimics for age-related macular degeneration treatment.
    Article Snippet: Age-related macular degeneration (AMD) is a leading cause of irreversible blindness in the elderly, and effective curative therapies are not available.. This study presents a tissue-engineering strategy for retinal regeneration using an injectable, in situ-forming hydrogel composed of thiol-functionalized hyaluronic acid (HA-SH) and polyvinyl alcohol (PVA), reinforced with peptide nanofibers.. Decellularized Bruch's membrane derived from goat eyes was used as a control and exhibited a fiber diameter of 75.09 ± 12.99 nm, thickness of 6.26 ± 1.12 μm, and hydrophilic properties (contact angle: 39.46 ± 0.46).

    Cell Culture:

    Article Title: Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumors
    Article Snippet: .. BON-1 cells were cultured in DMEM/F12; STC-1, HT-1080, and Hep G2 were cultured in DMEM; 786-O, QGP-1, and GOT-1 were cultured in ATCC-formulated RPMI-1640; Caki-1 was cultured in McCoy’s 5A. .. All media were supplemented with 10% fetal bovine serum (FBS) (Hyclone, Cytavia) and 1% penicillin/streptomycin (Gibco).

    Article Title: Immune escape mechanisms revealed by multifunctional nanoprobes targeting PD‐L1 in hepatic cancer progression
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense weremaintained in EMEM (30-2003, ATCC) with 10% FBS (Gibco, USA) and in RPMI-1640 (A1049101, Gibco) with 10% FBS plus 1% pen/strep (Gibco), respectively. .. The mouse normal liver cell line AML12 (CRL-2254, ATCC) was grown in DMEM/F12 (30-2006, ATCC) with 10%FBS, whereas themouseHCC cell linesHepa1-6 (CRL1830, ATCC) and H22 (CL-0341, Procell, Wuhan, China) were cultured in DMEM (11965092, Gibco) and RPMI-1640 (A1049101, Gibco), respectively, both supplemented with 10% FBS and 1% pen/strep. .. For co-culture experiments, isolated CD8+ T cells were seeded in the lower chamber of Transwell plates (CLS3412, Corning, USA), while HCC cells were placed in the upper chamber at a 1:5 ratio (HCC:CD8+ T cells).

    Article Title: FGF1 orchestrates circadian hepatic triglyceride secretion.
    Article Snippet: .. Cell Culture and Treatments AML12 (Alpha Mouse Liver 12) hepatocyte cell line purchased from the American Type Culture Collection (ATCC, CRL-2254) and cultured in DMEM-F12 (ATCC 30-2006) media supplemented with 10% Fetal Bovine Serum (FBS, Gibco A5256701), 1X Antibiotic-Antimycotic (GIBCO, 15240096), 1X Insulin (10 g/ml)-Transferrin (5.5 g/ml)Selenium (5 ng/ml) (ITS-G GibcoTM 51300044), and 40 ng/ml dexamethasone (Cell Signaling, 14776S). .. HepG2 human liver cancer cell line purchased from the American Type Culture Collection (ATCC, HB-8065) and cultured in EMEM (ATCC 30-2003) media supplemented with 10% Fetal Bovine Serum (FBS, Gibco A5256701), 1X Antibiotic-Antimycotic (GIBCO, 15240096).

    Article Title: FGF1 orchestrates circadian hepatic triglyceride secretion.
    Article Snippet: .. AML12 (Alpha Mouse Liver 12) hepatocyte cell line purchased from the American Type Culture Collection (ATCC, CRL-2254TM) and cultured in DMEM-F12 (ATCC 30-2006) media supplemented with 10% Fetal Bovine Serum (FBS, Gibco A5256701), 1X Antibiotic-Antimycotic (GIBCO, 15240096), 1X Insulin (10 g/ml)-Transferrin (5.5 g/ml)Selenium (5 ng/ml) (ITS-G GibcoTM 51300044), and 40 ng/ml dexamethasone (Cell Signaling, 14776S). .. HepG2 human liver cancer cell line purchased from the American Type Culture Collection (ATCC, HB-8065TM) and cultured in EMEM (ATCC 30-2003) media supplemented with 10% Fetal Bovine Serum (FBS, Gibco A5256701), 1X Antibiotic-Antimycotic (GIBCO, 15240096).

    Article Title: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells.
    Article Snippet: MCF-7 cells were cultured in EMEM (ATCC, 30-2003) supplemented with 10% FBS and 10 μg ml−1 insulin, and MDA-MB-231 cells were cultured in DMEM (ATCC, 30-2002) supplemented with 10% FBS. .. MCF-10A cells were cultured in DMEM-F12 (ATCC, 30-2006) supplemented with 5% horse serum (Millipore, H1138), 20 ng ml−1 EGF (Rockland, 009-001-C26), 0.5 mg ml−1 hydrocortisone (VWR, 1315004), 100 ng ml−1 cholera toxin (Millipore, 9012-63-9), 10 μg ml−1 insulin and 1% penicillin–streptomycin (VWR, 10220-718). ..

    Article Title: CHI3L1 Expression in Chordoma: Implications for Immunotherapeutic Intervention.
    Article Snippet: All commercial chordoma cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA) and seeded in T-75 flasks (Fisher Scientific Cat. 07-202-001, Waltham, MA, USA). .. JHC7 primary sacral chordoma cells were cultured in DMEM/F12 https://doi.org/10.3390/cells15040377 (ATCC Cat. 30-2006) media [55]; primary U-CH12 sacral chordoma cells in IMDM/RPMI (ATCC Cat. 30-2005/30-2001), L-glutamine (ATCC Cat. 30-2214), and ITS-G (Fisher Scientific Cat. 41-400-045) [56]; chordoma-derived U-CH1-N notochordal cells in Minimum Essential Medium Eagle (Sigma Aldrich StableCell Cat. M6074-500ML, St. Louis, MO, USA) media [57]; and U-CH1, recurrent sacrococcygeal chordoma cells, in IMDM/RPMI and L-glutamine, with collagen coating (Corning Cat. 354236, Corning, NY, USA) as per supplier instructions [58–62]. .. Media was supplemented with 10% fetal bovine serum (Cat. 30-2020) and 1% penicillin-streptomycin solution (Cat. 30-2300) from ATCC.

    Article Title: Timed secreted proteomes reveal regulation of hepatokines by the liver circadian clock.
    Article Snippet: .. Cell culture AML12 cells AML12 cells, purchased from ATCC (CRL-2254), were cultured in DMEM:F12 (ATCC, 30-2006) supplemented with 10% FBS (Corning, 35-015-CV), 1% penicillin-streptomycin, insulintransferrin-selenium supplement (Corning, 25-800-CR), and dexamethasone (40 ng/mL). .. Cells were plated at confluency of 40% in 96-well plate and the following day were transfected with the respective plasmids (Bmal1, Clock, Cry1, Cry2, fgb-luc, col18a1-luc, and scarmble-luc) at their corresponding doses according to the Lipofectamine 3000 Transfection Kit (Invitrogen, L3000001) with the exception of incubating the cells in OPTI-MEM for 4 hours after adding transfection reagents.



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